PCR genetic screening for presence of sea urchin DNA in rock lobster faecal samples
Data is PCR amplification results of southern rock lobster (Jasus edwardsii) faecal material tested for sea urchin DNA (using unique primers for Centrostephanus rodgersii and Heliocidaris erythrogramma) in an attempt to determine in situ rates of consumption of sea urchins by lobsters. An efficient and non-lethal method was used to source and screen lobster faecal samples for the presence of DNA from ecologically important sea urchins. Lobster faecal samples were collected from trap caught specimens sourced in winter & summer seasons over 2 years (2009-2011) within two no-take research reserves; declared specifically for the purpose of rebuilding large predatory-capable lobsters to assess the potential for predator-driven remediation of kelp beds on rocky reefs extensively overgrazed by sea urchins (North Eastern Tasmania) and reefs showing initial signs of overgrazing (South Eastern Tasmania). Data for molecular assays showed high variability in the proportion of lobsters testing positive to sea urchins, with significant variability detected across different years and seasons but this was found to vary depending on different lobster size-classes. Sea urchin DNA was also amplifiable from sediments and urchin faeces collected from the reef surface where urchins occurred in high abundance. Furthermore, positive sea urchin DNA assays were obtainable from lobster faeces after lobsteres were fed sediment and urchin faecal material.
Rates of predation obtained with genetics tests can also be compared to independent rates of urchin losses given known lobster abundances within research reserves (and at control sites). Data of changes in urchin abundances and lobster abundances are therefore also lodged as part of this record.
Simple
Identification info
- Date (Creation)
- 2011-09-16T15:58:00
Principal investigator
Collaborator
Collaborator
- Purpose
- Assessment of Management Options to Minimise Formation of 'Barrens' Habitat by the Long-Spined Sea Urchin (Centrostephanus rodgersii) in Tasmania
- Credit
- Funding: FRDC project 2007/045
- Status
- Completed
Principal investigator
Collaborator
- Spatial representation type
- Text, table
- Temporal resolution
-
P0Y6M0DT0H0M0S
- Topic category
-
- Biota
Extent
Temporal extent
- Time period
- 2008-04-01T00:00:00 2011-02-28T00:00:00
Vertical element
- Minimum value
- 3
- Maximum value
- 40
- Identifier
- EPSG::5715
- Name
- MSL depth
- Maintenance and update frequency
- Not planned
Resource format
- Title
- Microsoft Excel (xlsx)
- Date
- Edition
- 2007
- Keywords (Taxon)
-
- Centrostephanus rodgersii
- Jasus edwardsii
- Haliotis rubra
- Ecklonia radiata
- Heliocidaris erythrogramma
- AODN Keywords Thesaurus
-
- Genetics
- Urchin Barrens
- Kelp Beds
- DNA Detection
- Global Change Master Directory (GCMD) Earth Science Keywords Version 8.0
- Keywords (Discipline)
-
- Temperate Reef
- Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Resource constraints
- Classification
- Restricted
Resource constraints
- Use limitation
- The data described in this record are the intellectual property of University of Tasmania through the Institute for Marine and Antarctic Studies
Resource constraints
- Linkage
-
http://i.creativecommons.org/l/by-sa/2.5/au/88x31.png
License Graphic
- Title
- Creative Commons Attribution-Share Alike 2.5 Australia License
- Website
-
http://creativecommons.org/licenses/by-sa/2.5/au/
License Text
- Other constraints
- The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).
- Other constraints
- See point of contact for access to the data.
- Other constraints
- Use of this data is currently embargoed until publication in the peer-review literature.
Associated resource
- Title
- FRDC project 2007/045
- Date (unknown)
- 2011-09-16T00:00:00
- Language
- English
- Character encoding
- UTF8
- Supplemental Information
- References: Final FRDC Report in draft format.
Distribution Information
- Distribution format
-
- Microsoft Excel (xlsx)
- OnLine resource
- DATA - Genetic tests on lobster faeces [direct download]
- OnLine resource
- DATA - Genetic tests for sea urchin DNA in lobster faeces fed environmental and sea urchin faecal samples [direct download]
- OnLine resource
- DATA - Sea urchin density change within Research Reserves relative to control sites [direct download]
- OnLine resource
- README - data file help document [direct download]
- OnLine resource
-
imas:FR2_CRJohnson_Presence_sea_urchin_dna_in_rock_lobster_faecal_samples_GV
MAP - Collection sites for lobster faecal samples
Resource lineage
- Statement
- PCR detection of urchin DNA in lobster faecal material. Refer to "Data file help document” for general methods information, and for a full description of methods see the Draft Final Report of the Fisheries Research & Development Corporation research project #FRDC2007_045.
- Hierarchy level
- Dataset
Resource lineage
- Statement
- GPS used datum WGS84.
- Hierarchy level
- Collection hardware
Metadata
- Metadata identifier
- 665c701d-0737-4093-8ba0-93765fc12c94
- Language
- English
- Character encoding
- UTF8
Point of contact
Type of resource
- Resource scope
- Dataset
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/665c701d-0737-4093-8ba0-93765fc12c94
Point of truth URL of this metadata record
- Date info (Creation)
- 2020-09-17T17:43:27
- Date info (Revision)
- 2020-09-17T17:43:27
Metadata standard
- Title
- ISO 19115-3:2018