TemperateReefBase Geonetwork Catalogue
Macrocystis pyrifera thermal tolerance testing
Intraspecific variation in the thermal tolerance of microscopic giant kelp (Macrocystis pyrifera) sporophytes was tested using a common garden experiment, where 49 unique family-lines were raised under four different water temperatures (12, 16, 20, and 24°C). The unique family-lines were taken from ongoing giant kelp gametophyte cultures held at IMAS, and represented F1 offspring from seven 'selfed' individuals collected from 6 sites across ~250km in Tasmania, Australia, in addition to a site-level cross from each of the sites, and a panmictic cross using the 42 pure family lines.
Survivorship of the selected warm-adapted family-lines after outplanting trials at restoration sites can be found here with the associated dataset "NESP Marine Hub Project E7 outplanted kelp survivorship". https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/908afd8c-cc7a-4ea3-a87e-4497ae8da87a
Simple
Identification info
- Date (Revision)
- 2020-04-06
Principal investigator
Collaborator
- Credit
- Australian Government’s National Environmental Science Program (NESP) Marine Biodiversity Hub
- Status
- On going
Principal investigator
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Layton, Cayne
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7004
Australia
- Topic category
-
- Biota
Extent
N
S
E
W
))
Temporal extent
- Time period
- 2019-08-16
Vertical element
- Minimum value
- 1
- Maximum value
- 10
- Identifier
- EPSG::5715
- Name
- MSL depth
- Maintenance and update frequency
- As needed
- Keywords (Theme)
-
- seaweed
- macroalgae
- thermal tolerance
- temperature
- ocean warming
- Keywords (Taxon)
-
- Macrocystis pyrifera
- giant kelp
- Keywords (Discipline)
-
- Temperate Reef
- NASA/GCMD Keywords, Version 8.5
-
- EARTH SCIENCE | AGRICULTURE | AGRICULTURAL AQUATIC SCIENCES | AQUACULTURE
- EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | COASTAL | KELP FOREST
- EARTH SCIENCE | AGRICULTURE | AGRICULTURAL PLANT SCIENCE | PLANT BREEDING AND GENETICS
- EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | SPECIES/POPULATION INTERACTIONS | NATURAL SELECTION
- Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
- Keywords (Theme)
-
- individual (coded by site and replicate #)
- the source region of the parental material
- the source site of parental material.
- the type of cross used to fertilise the gametophytes and promote sporphyte developement.
- the testing temperature
- sporophyte abundance after the testing period
- average size of surviving sporophytes
Resource constraints
- Classification
- Unclassified
Resource constraints
- Linkage
-
http://i.creativecommons.org/l/by/4.0/88x31.png
License Graphic
- Title
- Creative Commons Attribution 4.0 International License
- Website
-
http://creativecommons.org/licenses/by/4.0/
License Text
- Other constraints
- The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).
- Language
- English
- Character encoding
- UTF8
- Supplemental Information
- Layton C & Johnson CR (2021). Assessing the feasibility of restoring giant kelp forests in Tasmania. Report to the National Environmental Science Program, Marine Biodiversity Hub. Institute for Marine and Antarctic Studies, University of Tasmania.
Content Information
- Content type
- Physical measurement
- Description
- These display the unique family-lines, which are coded by site (e.g. Site A, Site B...etc.) and individual (individual #1, #2...etc.). Also, 'cross' denotes a randomised of all family-lines from each site, while 'PAN' denotes the complete panmictic outcross of all family-lines.
- Name
- individual (coded by site and replicate #)
- Name
- indiv
- Description
- Only two regions present; a warmer 'north' region from northern Tasmania, and the cooler 'south' region from southeast Tasmania
- Name
- the source region of the parental material
- Name
- region
- Description
- There are 6 sites, which are named A-F in order from north to south.
- Name
- the source site of parental material.
- Name
- site
- Description
- 'self' denotes a selfed or inbred cross, whilst 'out' denotes an outcross.
- Name
- the type of cross used to fertilise the gametophytes and promote sporphyte developement.
- Name
- cross-type
- Description
- four temps; 12, 16, 20, 24C
- Name
- the testing temperature
- Name
- temp (C)
- Identifier
- http://vocab.nerc.ac.uk/collection/P06/current/UPAA
- Name
- Degrees Celsius
- Name
- sporophyte abundance after the testing period
- Name
- sporophyte abundance (count)
- Name
- count
- Description
- quantified from photographs.
- Name
- average size of surviving sporophytes
- Name
- size (mm2)
- Name
- mm2
Distribution Information
- Distribution format
-
- Microsoft Excel
- OnLine resource
- ASSOCIATED DATA - Outplanted kelp survivorship
Resource lineage
- Statement
- Intraspecific variation in thermal tolerance of microscopic sporophytes was tested using a common garden experiment, where 49 unique lines were raised under four different water temperatures (12, 16, 20, and 24°C). The common garden setup consisted of four temperature-controlled seawater baths, each containing the 49 family lines in individual 750 mL containers filled with seawater (containers were randomly positioned within each bath, and each bath randomly allocated a water temperature). All the baths were situated within a temperature-controlled room at 12°C, and the 16, 20, and 24°C seawater baths heated by aquarium heaters. All seawater used was 0.2μm filtered and UV sterilised, but had no added nutrients; in order to simulate ambient levels of nutrients from Tasmania. Each testing container was sealed except for a seawater inlet, a filtered air inlet, and an outlet. Aeration in each was achieved by constant gentle bubbling from a common air source, whilst every container within an individual seawater bath was connected to a single 25 L temperature-controlled header tank (i.e. one header tank per temperature treatment). Every ~72 hours the header tanks were opened, and each container received ~500 mL of new seawater (with the excess seawater overflowing from the outlet of each container). The family lines were introduced to the individual containers as gametophytes seeded onto a two microscopic slides, and fertilised at the common temperature of 12°C, to remove any effect of temperature on fertilisation success. At this initial stage, each water bath was illuminated with blue fluorescent lighting on a 12:12 light/dark cycle for 14 days, followed by an additional 14 days of illumination under blue and white light fluorescent lighting. Following development to the very early sporophyte stage over this period, the temperature in each bath was adjusted to the experimental temperature at a rate of ~2°C every 24 hours (thus the 24°C water bath took 6 days to reach the experimental temperature). At this point, the experiment was considered to have begun. After this period, sporophytes were allowed to develop and grow for ~40 days. One microscope slide was then taken each container, and the sporophytes counted and photographed under a microscope from a standardised central portion of the slide.
- Hierarchy level
- Dataset
Metadata
- Metadata identifier
- 0b91d7fd-7d29-452f-954a-78cf75151035
- Language
- English
- Character encoding
- UTF8
Point of contact
- Parent metadata
Type of resource
- Resource scope
- Dataset
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/0b91d7fd-7d29-452f-954a-78cf75151035
Point of truth URL of this metadata record
- Date info (Creation)
- 2021-04-08T17:28:53
- Date info (Revision)
- 2021-04-08T17:28:53
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
N
S
E
W
Provided by
Associated resources
Not available