TemperateReefBase Geonetwork Catalogue
Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems
Our aim was to compare water and sediment as sources of environmental DNA (eDNA) to better characterise Antarctic benthic communities and further develop practical approaches for DNA-based biodiversity assessment in remote environments. We used a cytochrome c oxidase subunit I (COI) metabarcoding approach to characterise metazoan communities in 26 nearshore sites across 12 locations (including Ellis Fjord, Warriner Channel, Hawker Channel, Abatus Bay, Powell Point, Shirokaya Bay, and Weddell Arm) in the Vestfold Hills (East Antarctica) based on DNA extracted from either sediment cores or filtered seawater. We detected a total of 99 metazoan species from 12 phyla (including nematodes, cnidaria, echinoderms, chordates, arthropods, annelids, rotifers and molluscs) across 26 sites, with similar numbers of species detected in sediment and water eDNA samples.
Please cite: Clarke LJ et al. (2021). Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems. PeerJ, DOI: 10.7717/peerj.12458
This work was completed as part of the Davis Aerodrome Project (DAP).
Simple
Identification info
- Alternate title
- Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems
- Date (Publication)
- 2022-06-30
- Edition
- 1
- Citation identifier
-
Dataset DOI
- Title
- Information and documentation - Digital object identifier system
- Date (Publication)
- 2012-04-23
- Citation identifier
- ISO 26324:2012
- Citation identifier
- doi:10.26179/caaz-b854
Originator
Clarke, L., Suter, L., Polanowski, A., Deagle, B., Stark, J. and Johnstone, G.J.
Publisher
Australian Antarctic Data Centre
Principal investigator
Principal investigator
Principal investigator
POLANOWSKI, ANDREA
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Principal investigator
DEAGLE, BRUCE
CSIRO Australian National Fish Collection (ANFC)
Hobart
Tasmania
Hobart
Tasmania
Australia
Principal investigator
Principal investigator
JOHNSTONE, GLENN JAMES
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Collaborator
Collaborator
Collaborator
JOHNSTONE, GLENN JAMES
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
- Name
- CAASM Metadata
- Other citation details
- Unreleased data
- Purpose
- To explore the potential of environmental DNA (eDNA) as a metazoan biodiversity monitoring tool for Antarctic benthic ecosystems. Specifically: (1) Are there differences between sediment and water eDNA samples in terms of (a) number of reads assigned to metazoans, non-metazoans, and unclassified, (b) the number of metazoan species detected, (c) metazoan community composition? (2) Does eDNA-based species richness decrease with sediment depth, similar to trends observed in morphology-based studies? (3) Do environmental parameters (e.g., depth, distance to open ocean) influence community composition in water eDNA?
- Status
- Completed
Custodian
AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
- Topic category
-
- Oceans
- Biota
Extent
N
S
E
W
))
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2019-11-13 2019-12-14
Extent
- Description
- Vertical Extent - Depth
Vertical element
- Minimum value
- 3.6
- Maximum value
- 39
- Identifier
- urn:x-ogc:def:cs:EPSG:6498
- Name
- Vertical CS. Axis: depth (D). Orientation: down. UoM: m.
- Identifier
- urn:x-ogc:def:axis:EPSG:113
- Name
- depth
- Title
- Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems.
- Date (Publication)
- 2021
- Citation identifier
- doi:10.7717/peerj.12458
Author
Clarke LJ et al.
- Name
- PeerJ
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > MOLLUSKS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ROTIFERS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > SEGMENTED WORMS (ANNELIDS)
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ECHINODERMS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > CNIDARIANS
- EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC
- Keywords
-
- ENVIRONMENTAL DNA
- DNA METABARCODING
- CYTOCHROME C OXIDASE SUBUNIT I (COI)
- METAZOAN
- NEMATODES
- CNIDARIA
- ECHINODERMS
- CHORDATES
- ARTHROPODS
- ANNELIDS
- ROTIFERS
- MOLLUSCS
- ILLUMINA MISEQ
- NASA/GCMD Earth Science Keywords
-
- ADS > Automated DNA Sequencer
- NASA/GCMD Earth Science Keywords
-
- LABORATORY
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- AMD
- CEOS
- NASA/GCMD Earth Science Keywords
-
- GEOGRAPHIC REGION > POLAR
- CONTINENT > ANTARCTICA > VESTFOLD HILLS
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- These data are not yet publicly available for download.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_5097_DAP_nearshore_benthic_DNA_metabarcoding when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Distributor
- Fees
- Free
- Planned available datetime
- 2022-06-30T00:00:00
- Units of distribution
- MB
- Transfer size
- 2.9
- Distribution format
-
- Excel, csv, FASTA, Word
- OnLine resource
-
GET DATA > DIRECT DOWNLOAD
Download the dataset.
- OnLine resource
-
PROJECT HOME PAGE
Public information for AAS project 5097
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset.
Resource lineage
- Statement
- Only a small proportion of DNA sequences were assigned to the target taxa (multicellular animals, or metazoa). Samples that yielded less than 1000 metazoan DNA sequence reads are unlikely to provide sufficient DNA sequence depth to properly characterise metazoan diversity in those samples, and were excluded. Non-invasive, “capture-free” approaches like eDNA also raise the question: how do we know an organism detected with eDNA is really there? The issue is discussed in the final paragraph of the article.
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
- Maintenance note
- 2021-11-26 - record created by Laurence Clarke.
Metadata
- Metadata identifier
- string/AAS_5097_DAP_nearshore_benthic_DNA_metabarcoding
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Australian Antarctic Division
Owner
AADC
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 27a4ef6e-fce1-4106-b7bc-23eee783e003
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2021-11-29T10:33:42
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/AAS_5097_DAP_nearshore_benthic_DNA_metabarcoding
Point of truth for the metadata record
- Date info (Creation)
- 2021-11-26T00:00:00
- Date info (Last Update)
- 2021-11-29
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile
Overviews
Spatial extent
N
S
E
W
Provided by
Associated resources
Not available