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Phenotypic plasticity in habitat-forming seaweeds subject to latitudinal transplant

The phenotypic plasticity of habitat-forming seaweeds was investigated with a transplant experiment in which juvenile Ecklonia radiata and Phyllospora comosa were transplanted from NSW (warm conditions) to Tasmania (cool conditions) and monitored for four months. We used multiple performance indicators (growth, photosynthetic characteristics, pigment content, chemical composition, stable isotopes, nucleic acids) to assess the ecophysiology of seaweeds before and following transplantation between February 2012 and June 2012.

Simple

Identification info

Date (Creation)
2016-02-22

Identifier

Title
Information and documentation - Digital object identifier system
Citation identifier
ISO 26324:2012

Code
10.4226/77/572171cf5940f
Codespace
doi.org
Description
Digital Object Identifier (DOI)

Principal investigator

Institute for Marine and Antarctic Studies - Flukes, Emma (Principal investigator)
Australia
ROR ID >

ORCID >

Collaborator

Institute for Marine and Antarctic Studies - Johnson, Craig (Primary supervisor)
Australia
ROR ID >

ORCID >

Collaborator

Institute for Marine and Antarctic Studies - Wright, Jeffrey
Australia
ROR ID >

ORCID >

Credit
Australian Research Council (ARC) grant DP1096573
Status
Completed

Point of contact

Institute for Marine and Antarctic Studies - Flukes, Emma
Private Bag 129
Hobart
Tasmania
7001
Australia
ROR ID >

ORCID >

Temporal resolution
P0Y1M0DT0H0M0S
Topic category
  • Biota

Extent

N
S
E
W


N
S
E
W


Temporal extent

Time period
2012-02-25 2012-06-30

Vertical element

Minimum value
5
Maximum value
10
Identifier
EPSG:5715
Name
MSL depth
Maintenance and update frequency
Not planned

Resource format

Title
CSV
Date
Edition
n/a
Keywords (Theme)
  • phycology
  • kelp
  • PAM fluorometry
  • ecophysiology
  • RNA:DNA
  • transplantation
  • ecosystem engineer
  • phenotypic plasticity
  • in situ measurements
Keywords (Taxon)
  • Ecklonia radiata
  • Phyllospora comosa
NASA/GCMD Keywords, Version 8.5
  • EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL
  • EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS)
  • EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE
  • EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS
  • EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS
  • EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF
  • EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC
Keywords (Discipline)
  • Temperate Reef
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
  • Ecological Physiology
  • Phycology (incl. Marine Grasses)
  • Marine and Estuarine Ecology (incl. Marine Ichthyology)
  • Ecological Impacts of Climate Change
AODN Platform Vocabulary
  • diver
AODN Instrument Vocabulary
  • fluorometers
  • high performance liquid chromatographs
  • spectrophotometers
  • gas chromatograph mass spectrometers

Resource constraints

Classification
Unclassified

Resource constraints

Linkage
http://i.creativecommons.org/l/by/4.0/88x31.png

License Graphic

Title
Creative Commons Attribution 4.0 International License


>

Website
http://creativecommons.org/licenses/by/4.0/

License Text

Other constraints
Cite data as: Flukes, E., Johnson, C., & Wright, J. (2016). Phenotypic plasticity in habitat-forming seaweeds subject to latitudinal transplant [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.4226/77/572171CF5940F
Other constraints
The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.
Language
English
Character encoding
UTF8

Content Information

Content type
Physical measurement
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Relative electron transport rate

Name
rETRmax

Name
no units (relative units)
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Alpha

Name
Alpha

Name
nil
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Minimum saturating irradiance

Name
Ek

Name
µmol photons m-2 s-1
Description
measured using blue diving-PAM fluorometer (contact researcher for PAM settings)
Name
Maximum quantum yield (dark-acclimated sample) - also Fv/Fm

Name
Fv.Fm

Name
nil (ratio)
Name
Tissue concentration of fucoxanthin

Name
Fucoxanthin

Name
mg g-1 wet weight
Name
Tissue concentration of chlorophyll a

Name
ChlA

Name
mg g-1 wet weight
Name
Tissue concentration of chlorophyll c

Name
ChlC

Name
mg g-1 wet weight
Description
Both RNA and DNA concentrations were extracted from a single sample of tissue
Name
Ratio of tissue RNA to DNA concentration

Name
RNA.DNA

Name
nil (ratio)
Description
measured using extraction method developed specifically for this data
Name
Tissue concentration of RNA

Name
RNA

Name
µg g wet weight-1
Description
measured using extraction method developed specifically for this data
Name
Tissue concentration of DNA

Name
DNA

Name
µg g wet weight-1
Name
Tissue percentage carbon

Name
per_C

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPCT
Name
Percent
Name
Tissue percentage nitrogen

Name
per_N

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UPCT
Name
Percent
Description
measured using mass spectrometry
Name
Ratio of tissue carbon to nitrogen

Name
C.N

Name
nil (ratio)
Description
Pee Dee Belemnite as standard
Name
δ13C

Name
d13C

Name
‰
Description
Air as standard
Name
δ15N

Name
d15N

Name
‰
Description
measured as length x width of blades/lamina
Name
Blade area

Name
Blade_area

Name
mm^2

Distribution Information

Distribution format
  • CSV (.csv)

OnLine resource
DATA - Ecophysiological measurements prior to, and 4 months following transplantation (.csv) [direct download]

OnLine resource
DATA - Growth of seaweeds at monthly intervals (.csv) [direct download]

OnLine resource
DATA - Raw PAM fluorometry RLC outputs at monthly intervals (zipped .csv) [direct download]

OnLine resource
ASSOCIATED PUBLICATION - Ecophysiology of habitat-forming seaweeds in a changing environment [PhD thesis]

See Chapter 2

Resource lineage

Statement
Juvenile Ecklonia radiata and Phyllospora comosa were transplanted from NSW-Tas and Tas-Tas. Seaweeds were first collected and kept overnight in the laboratory where they were attached to lengths of 6 mm diameter polypropylene rope by inserting holdfasts into the weave of the rope. Phyllospora were outplanted at a depth of ~5m and Ecklonia at ~10m in Fortescue Bay, Tasmania on 28th/29th February 2012. Measurements of growth and PAM fluorometry were made monthly, while other destructive ecophysiological measurements were made in situ at the time of collection, and 123 days following transplantation. The following parameters were measured: growth, PAM fluorometry photosynthetic characteristics, pigment content (chlorophyll a, c, fucoxanthin), tissue chemistry (δ13C, δ15N, % C, % N, C:N ratios), and nucleic acid characteristics (absolute RNA and DNA, RNA:DNA ratios). Terminal measurements were made on 30th June 2012. Pigments were measured using spectrophotometry and HPLC. Tissue chemistry was measured using mass spectrometry. Nucleic acids were measured using a novel technique described in Flukes et al. 2016. See Flukes et al. 2016 for a full description of all methodologies. NOTE: in data file "otherparamdata.csv", "start" sampling refers to in situ samples, "end" sampling refers to terminal measurements at t = 123 days. In data files "growthdata.csv" and contents of "raw_PAM_RLC_output", sample times refer to time since transplantation (days).
Hierarchy level
Dataset
Hierarchy level
Dataset

Platform

Identifier

Code
diver

Instrument

Identifier

Code
fluorometers
Hierarchy level
Dataset

Instrument

Identifier

Code
high performance liquid chromatographs
Hierarchy level
Dataset

Instrument

Identifier

Code
spectrophotometers
Hierarchy level
Dataset

Instrument

Identifier

Code
gas chromatograph mass spectrometers

Metadata

Metadata identifier
urn:uuid/6cbf0c73-1ade-4c1d-a83f-555118cf15ea

Language
English
Character encoding
UTF8

Author

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager

Type of resource

Resource scope
Dataset
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/6cbf0c73-1ade-4c1d-a83f-555118cf15ea

Point of truth URL of this metadata record

Date info (Creation)
2020-08-14T12:02:23
Date info (Revision)
2025-01-19T20:30:53

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

N
S
E
W


N
S
E
W


Keywords

PAM fluorometry RNA:DNA ecophysiology ecosystem engineer in situ measurements kelp phenotypic plasticity phycology transplantation
AODN Platform Vocabulary
diver
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Ecological Impacts of Climate Change Ecological Physiology Marine and Estuarine Ecology (incl. Marine Ichthyology) Phycology (incl. Marine Grasses)
NASA/GCMD Keywords, Version 8.5
EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | MACROALGAE (SEAWEEDS) | BROWN ALGAE EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | BENTHIC EARTH SCIENCE | BIOSPHERE | ECOSYSTEMS | MARINE ECOSYSTEMS | REEF EARTH SCIENCE | BIOSPHERE | VEGETATION | CHLOROPHYLL EARTH SCIENCE | BIOSPHERE | VEGETATION | PIGMENTS

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