Pigment sampling in the coastal waters of south eastern Tasmania

Water samples for the analysis of pigments using High Performance Liquid Chromatography (HPLC) were collected only in the first 12 months of the sampling program. Pigment analysis is used to estimate algal community composition and concentration. Pigments which relate specifically to an algal class are termed marker or diagnostic pigments. Some of these diagnostic pigments are found exclusively in one algal class (e.g. prasinoxanthin in prasinophytes), while others are the principal pigments of one class, but are also found in other classes (e.g. fucoxanthin in diatoms and some haptophytes; 19′-butanoyloxyfucoxanthin in chrysophytes and some haptophytes). The presence or absence of these diagnostic pigments can provide a simple guide to the composition of a phytoplankton community, including identifying classes of small flagellates that cannot be determined by light microscopy techniques. There was general similarity in pigment composition between all sites, with a presence of diatoms (as indicated by fucoxanthin), haptophytes (hex-fucoxanthin), prasinophytes (prasinoxanthan), cryptophytes (alloxanthan), cyanophytes (zeaxanthan) and green algae (chl-b) in nearly all monthly samples at all sites. The green algae could be in the form of euglenophytes or prasinophytes; the absence of the pigment lutein in all samples indicates that chlorophytes are not present in Storm Bay, at least at the sites sampled.

Simple

Identification info

Date (Creation)
2018-06-25

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Swadling, Kerrie, Dr
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7001
Australia
ORCID ID >

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Eriksen, Ruth, Dr
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7001
Australia
ORCID ID >

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Beard, Jason, Mr
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7001
Australia
ORCID ID >

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Crawford, Christine, Dr
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7001
Australia
ORCID ID >

Credit
Fisheries Research and Development Corporation (FRDC)
Status
Completed

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Swadling, Kerrie, Dr
IMAS - Hobart
Private Bag 129
Hobart
Tasmania
7001
Australia
ORCID ID >

Temporal resolution
P0Y1M0DT0H0M0S
Topic category
  • Biota

Extent

N
S
E
W


Temporal extent

Time period
2009-11-01 2015-04-30

Vertical element

Minimum value
0
Maximum value
90
Identifier
EPSG::5715
Name
MSL depth
Maintenance and update frequency
Not planned
NASA/GCMD Keywords, Version 8.5
Keywords (Place)
  • Storm Bay
Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Keywords (Discipline)
  • Temperate Reef
AODN Platform Vocabulary
AODN Discovery Parameter Vocabulary
Keywords (Theme)
  • Pigment description
AODN Instrument Vocabulary

Resource constraints

Classification
Unclassified

Resource constraints

Use limitation
The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies. Please contact Kerrie Swadling (Principal Investigator) if you wish to use the data.

Resource constraints

Linkage
http://i.creativecommons.org/l/by/4.0/88x31.png

License Graphic

Title
Creative Commons Attribution 4.0 International License


>

Website
http://creativecommons.org/licenses/by/4.0/

License Text

Other constraints
Swadling, K.M., Eriksen, R.S., Beard, J.M. and Crawford, C.M. (2018) Phytoplankton sampling in the coastal waters of south eastern Tasmania. Institute for Marine and Antarctic Studies, University of Tasmania. Data accessed at http://metadata.imas.utas.edu.au/geonetwork/srv/en/metadata.show?uuid=42a88891-570f-4def-b4d5-07ccf7bba269 on (access date).
Other constraints
Please contact Kerrie Swadling (Principal Investigator) if you wish to use the data.

Associated resource

Title
East Coast Plankton
Alternate title
ECP
Date
Association Type
Larger work citation
Language
English
Character encoding
UTF8
Supplemental Information
Swadling, K.M., Eriksen, R.S., Beard, J.M. and Crawford, C.M. Institute for Marine and Antarctic Studies, 2017, Salmon Sub-program: Marine currents, nutrients and plankton in the coastal waters of south eastern Tasmania and responses to changing weather patterns, Hobart, Tasmania, June. CC BY 3.0

Content Information

Content type
Physical measurement

Identifier

Code
Downwelling vector irradiance as photons (PAR wavelengths) in the water body
Name
LICOR_AV

Identifier
http://vocab.aodn.org.au/def/unitsofmeasure/entity/585
Name
Micromoles per second per square metre

Identifier

Code
Sea-floor depth below surface of the water body
Name
DEPTH

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/ULAA
Name
Metres

Identifier

Code
Secchi depth
Name
DEPTH_SECCHI

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/ULAA
Name
Metres
Name
Pigment description

Name
PIGMENT

Identifier

Code
Concentration of total pigment per unit volume of the water body
Name
CONCENTRATION

Identifier
http://vocab.nerc.ac.uk/collection/P06/current/UGPL
Name
Micrograms per litre

Distribution Information

Distribution format
  • CSV

OnLine resource
DATA ACCESS - Pigment sampling [direct download]

OnLine resource
PUBLICATION - Salmon Sub-program: Marine currents, nutrients and plankton in the coastal waters of south eastern Tasmania and responses to changing weather patterns.

OnLine resource
imas:ECP_GEOSERVER_PIGMENT

MAP - Pigment sampling locations

OnLine resource
imas:ECP_GEOSERVER_PIGMENT

This OGC WFS service returns the data in subsettable CSV format.

OnLine resource
View and download this data through the interactive IMAS Data Portal.

Resource lineage

Statement
An integrated water column sample (12 m) using a weighted hose was collected for phytoplankton speciation and pigment analysis from February 2010. Prior to this, samples were collected from 1 m below the surface. Following the decision to include the integrated sampler, the sampling regime was revised in the following way. The contents of four sampling tube collections were mixed together and sub-sampled: duplicate 1 L samples for pigments were kept cold and dark until processing in the laboratory on return to shore. Water samples were also collected, using a 6 L or 8 L Niskin bottle, from 0.5 – 1 m below the surface, at 10 m depth, and within 5 m of the seabed. At site 3 an intermediate sample from 50 m was also collected. One to two litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) under subdued lighting, and the filter was then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mL) in a 10 mL centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated in an ice-water bath for 15 minutes in the dark. The samples were kept in the dark at 4°C for approximately 15 hours. After this time 200 mL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more in an ice-water bath for 15 minutes. The extracts were quantitatively transferred to a clean centrifuge tube and centrifuged to remove the filter paper. The final extract was filtered through a 0.2 µm membrane filter (Advantec MFS) prior to analysis by HPLC using a Waters–Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated auto sampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10, 28 mM tetrabutyl ammonium acetate, pH 6.5 methanol) within the sample loop. After injection, pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) and a binary gradient elution procedure. The flow rate was 1.1 mL min-1 and the column temperature was 55 oC. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b and ,-carotene in sample chromatograms were determined from standards (Sigma, USA) while all other pigment concentrations were determined from standards (DHI, Denmark).
Hierarchy level
Dataset
Hierarchy level
Dataset

Platform

Identifier

Code
research vessel
Hierarchy level
Dataset

Instrument

Identifier

Code
LI-COR LI-192 PAR sensor

Metadata

Metadata identifier
42a88891-570f-4def-b4d5-07ccf7bba269

Language
English
Character encoding
UTF8

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager
Parent metadata

Type of resource

Resource scope
Dataset
Metadata linkage
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/42a88891-570f-4def-b4d5-07ccf7bba269

Point of truth URL of this metadata record

Date info (Creation)
2019-03-06T17:31:28
Date info (Revision)
2019-03-06T17:31:28

Metadata standard

Title
ISO 19115-3:2018
 
 

Overviews

Spatial extent

N
S
E
W


Keywords

Pigment description
AODN Discovery Parameter Vocabulary
Concentration of total pigment per unit volume of the water body Downwelling vector irradiance as photons (PAR wavelengths) in the water body Sea-floor depth below surface of the water body Secchi depth
NASA/GCMD Keywords, Version 8.5
EARTH SCIENCE | AGRICULTURE | AGRICULTURAL AQUATIC SCIENCES | AQUACULTURE EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | FLAGELLATES | DINOFLAGELLATES EARTH SCIENCE | OCEANS | OCEAN CHEMISTRY | PIGMENTS | CHLOROPHYLL EARTH SCIENCE | OCEANS | OCEAN CIRCULATION | OCEAN CURRENTS EARTH SCIENCE | TERRESTRIAL HYDROSPHERE | WATER QUALITY/WATER CHEMISTRY

Provided by

Share on social sites

Access to the record in catalogue

Associated resources

Not available